Cell sorting (FACS)

Cell sorting (FACS)

A sophisticated method for the characterization and isolation of subpopulations of cells identified by multiparameter analysis

The facility provides a wide range of flow cytometric techniques that allow various experimental approaches to research problems. We operate one analytical instrument and three preparative cell sorters from Becton Dickinson and SONY, and offer a service that is comprehensive, flexible and user-friendly, using high-end, state-of-the-art methodologies. We also provide training, assistance and technical support, including advice on the experimental design, sample handling, data collection, analysis and interpretation.

Contact
Hubertus Kohler: Platform head, cell sorting

Obradovic MMS, Hamelin B, Manevski N, Couto JP, Sethi A, Coissieux MM, Münst S, Okamoto R, Kohler H, Schmidt A, Bentires-Alj M (2019) Glucocorticoids promote breast cancer metastasis.

Nature. 2019 Mar;567(7749):540-544

Moncayo G, Grzmil M, Smirnova T, Zmarz P, Huber RM, Hynx D, Kohler H, Wang Y, Hotz HR, Hynes NE, Keller G, Frank S, Merlo A, Hemmings BA (2018) SYK inhibition blocks proliferation and migration of glioma cells and modifies the tumor microenvironment.

Neuro Oncol. 2018 Apr 9;20(5):621-631

Daum JM, Keles Ö, Holwerda SJ, Kohler H, Rijli FM, Stadler M, Roska B (2017) The formation of the light-sensing compartment of cone photoreceptors coincides with a transcriptional switch.

Elife. 2017 Nov 6;6

Minoux M, Holwerda S, Vitobello A, Kitazawa T, Kohler H, Stadler MB, Rijli FM (2017) Gene bivalency at Polycomb domains regulates cranial neural crest positional identity.

Science. 2017 Mar 31;355(6332)

Koren S, Reavie L, Couto JP, De Silva D, Stadler MB, Roloff T, Britschgi A, Eichlisberger T, Kohler H, Aina O, Cardiff RD, Bentires-Alj M (2015) PIK3CA(H1047R) induces multipotency and multi-lineage mammary tumours.

Nature. 2015 Sep 3;525(7567):114-8

Cronin T, Vandenberghe LH, Hantz P, Juttner J, Reimann A, Kacsó AE, Huckfeldt RM, Busskamp V, Kohler H, Lagali PS, Roska B, Bennett J (2014) Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno-associated virus capsid and promoter.

EMBO Mol Med. 2014 Sep;6(9):1175-90

Du Roure C, Versavel A, Doll T, Cao C, Pillonel V, Matthias G, Kaller M, Spetz JF, Kopp P, Kohler H, Müller M, Matthias P (2014) Hematopoietic overexpression of FOG1 does not affect B-cells but reduces the number of circulating eosinophils.

PLoS One. 2014;9(4):e92836

Yokobayashi S, Liang CY, Kohler H, Nestorov P, Liu Z, Vidal M, van Lohuizen M, Roloff TC, Peters AH (2013) PRC1 coordinates timing of sexual differentiation of female primordial germ cells.

Nature. 2013 Mar 14;495(7440):236-40

Milani ES, Brinkhaus H, Dueggeli R, Klebba I, Mueller U, Stadler M, Kohler H, Smalley MJ, Bentires-Alj M (2013) Protein tyrosine phosphatase 1B restrains mammary alveologenesis and secretory differentiation.

Development. 2013 Jan 1;140(1):117-25

Lai JC, Ponti S, Pan D, Kohler H, Skoda RC, Matthias P, Nagamine Y (2012) The DEAH-box helicase RHAU is an essential gene and critical for mouse hematopoiesis.

Blood. 2012 May 3;119(18):4291-300

Siegert S, Cabuy E, Scherf BG, Kohler H, Panda S, Le YZ, Fehling HJ, Gaidatzis D, Stadler MB, Roska B (2012) Transcriptional code and disease map for adult retinal cell types.

Nat Neurosci. 2012 Jan 22;15(3):487-95, S1-2

Schwaiger M, Kohler H, Oakeley EJ, Stadler MB, Schübeler D (2010) Heterochromatin protein 1 (HP1) modulates replication timing of the Drosophila genome.

Genome Res. 2010 Jun;20(6):771-80

Yamaguchi T, Cubizolles F, Zhang Y, Reichert N, Kohler H, Seiser C, Matthias P (2010) Histone deacetylases 1 and 2 act in concert to promote the G1-to-S progression.

Genes Dev. 2010 Mar 1;24(5):455-69

Schwaiger M, Stadler MB, Bell O, Kohler H, Oakeley EJ, Schübeler D (2009) Chromatin state marks cell-type- and gender-specific replication of the Drosophila genome.

Genes Dev. 2009 Mar 1;23(5):589-601

Bordon A, Bosco N, Du Roure C, Bartholdy B, Kohler H, Matthias G, Rolink AG, Matthias P (2008) Enforced expression of the transcriptional coactivator OBF1 impairs B cell differentiation at the earliest stage of development.

PLoS One. 2008;3(12):e4007

Members

Platform/Service Head

Hubertus Kohler (Platform head, cell sorting)

Alumni

Biography of Hubertus Kohler

Positions held

2002-
Head FACS Facility, Friedrich Miescher Institute for Biomedical research, Basel Switzerland
1999-2002
Head FACS Facility, Basel Institute for Immunology and Basilea Pharmaceutica AG, Switzerland
1990-1999
Cytometry Specialist FACS Facility, Max-Planck-Institute of Immunobiology and Epigenetics, Freiburg i.Br. Germany

Software and Tools

Test - Not in use for now